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Related post: States among certain "high-risk populations (male homosexuals, illicit drug users, Haitians, and, to a lesser extent, hemophiliacs, recipients of blood transfusions and intimate contacts of cases). The epidemiology of the syndrome suggests that it is caused by a transmissible agent. Attempts to transmit a putative AIDS agent to chimpanzees and monkeys are in progress. 22-40 PHS 6040 (Rev. 1/83) GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00322-02 LID PERIOD COVERED October 1, 1982 through September 30, 1983 TITLE OF PROJECT (80 charactere or less. Title must fit on one line between the borders.) SEQUENCE ANALYSIS OF RESPIRATORY SYNCYTIAL (RS) VIRUS NUCLEOCAPSID GENE PRINCIPAL INVESTIGATOR (List other prof eesional personnel on subsequent pages.) (Name, title, laboratory, and Institute affiliation) S. Venkatesan, M.D. Expert, Respiratory Viruses Section LID, NIAID COOPERATING UNITS (if any) LAB/BRANCH LABORATORY OF INFECTIOUS DISEASES SECTION RESPIRATORY VIRUSES SECTION INSTITUTE AND LOCATION NIAID, NIH, BETHESDA, MARYLAND 20205 TOTAL MANYEARS: 0.9 PROFESSIONAL: 0.7 OTHER: 0.2 CHECK APPROPRIATE BOX(ES) CZ] (a) Human subjects □ (a1) Minors □ (a2) Interviews □ (b) Human tissues Lxl (c) Neither SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.) Recently, we obtained several cDNA clones encoding the RS viral nucleocapsid protein (NC), matrix protein (M), phosphoprotein (P) and a nonstructural protein (NSp) (Venkatesan et aj_. , PNAS, 1983). We have since sequenced a cDNA plasmid encoding the entire coding sequence of the NC gene. The 3' terminus of this gene has been identified by the presence of 14 poly A residues at one end of the clone. The cloned DNA lacks 6 nucleotides of the 5' end of the mRNA as determined by primer extension and gene walking on the mRNA. However, a single open reading frame of 1412 nucleotides encoding a protein of 467 amino acids is present within the cloned DNA. This translated protein of 51540 daltons is rich in basic amino acids, relatively rich in proline, but poor in cysteine. It has no sequence homology with the capsid proteins of other negative strand RNA viruses implying that RS virus is evolutionarily distinct. Interestingly, the sequence upstream of the Buy Lamprene poly A tail of this gene was not homologous to a similar region in the other RS viral genes. 22-41 PHS 6040 (Rev. 1/83) GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00368-01 LID PERIOD COVERED October 1, 1982 through September 30, 1983 TITLE OF PROJECT (80 charocteri or leu. Title mutt fit on one tine between the borden.) SEQUENCE ANALYSIS OF THE 3' END(s) OF RS VIRAL AND PARAINFLUENZA GENOME PRINCIPAL INVESTIGATOR (Litt other profeuional personnel on »ub>equent pagee.) (Nome, title, laboratory, and inititute affiliation) S. Venkatesan, M.D. Expert, Respiratory Viruses Section LID, NIAID COOPERATING UNITS (if any) LAB/BRANCH LABORATORY OF INFECTIOUS DISEASES SECTION RESPIRATORY VIRUSES SECTION INSTITUTE AND LOCATION NIAID, NIH, BETHESDA, MD 20205 TOTAL MANYEARS: 0.5 PROFESSIONAL: 0.2 OTHER; 0.3 CHECK APPROPRIATE BOX(ES) I I (a) Human subjects □ (al) Minors □ (a2) Interviews □ (b) Human tissues Cxi (c) Neither SUMMARY OF WORK (Use standard unreduced type. Do not exeeed the space provided.) RS virus despite its poor growth in vitro , has been grown to high titer by us and this has allowed us to purify virus by successive cycles of sucrose gradient centrifugation. In addition to genomic 50S RNA, purified virus preparations contain cellular RNA contaminants. The 50S genomic RNA was purified away from these contaminants and 3' end labeled by standard
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